Eurotransplant Reference Laboratory
Information about Haplotype generator
Haplotype generator.
The Batch calculator is an application to be used by professionals working in HLA Laboratories and other HLA specialists to determine haplotypes. The application can define the panel % Panel Reactive Antibodies (PRA) within the Eurotransplant donor population, determine the chance of a patient to receive an offer of a compatible organ transplant within the Eurotransplant donor population and determine the chance of a highly sensitized patient to receive an offer of a compatible renal transplant when included in the Eurotransplant Acceptable Mismatch (AM) program.
The program uses moleculair typing entered according to the official HLA nomenclature.

Description of the underlying database.
Calculations are performed on HLA data of deceased donors used for renal transplantation between 2010-2014 within the Eurotransplant area.
Donors with complete HLA typing for HLA-A, -B, -C, -DR and -DQ have been selected. Donors with missing HLA data for one or more alleles were excluded after verification that the HLA antigens that were known from these donors were not significantly different from the HLA antigens of donors with complete HLA information.
The total number of datasets from donors in the database is 6870, with all loci being in Hardy-Weinberg Equilibrium on broad level.
All alleles are available at split level, except B14, Cw3 and DQ3. Bw4 and Bw6 are included in the database. In case of missing data, Bw4/Bw6 has been assigned based on B-antigens.
Please be aware that assigning a Bw4 will not include corresponding HLA-A antigens. HLA-A antigens harbouring a Bw4 epitope need to be filled in separately.
Finally, DR51/52/53 are also included in the database. In case of missing data, imputation based on DRB1 typing was performed. DR53 has been removed for donors with DR7 and DQ9, because in this case DR53 is non-expressed.


A technical description for ICT professionals can be found here: ETRL batch calculator user guide.

For any suggestion or remarks, please contact:
etrl@eurotransplant.org